Regulatory

Part:BBa_K112321:Design

Designed by: Aron Lau   Group: iGEM08_UC_Berkeley   (2008-10-22)


{H-NS!} using MG1655 reverse oligo in BBb format


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR JH088F/JHO88R on Salmonella L2.     (435bp, EcoRI/BamHI/DpnI)
Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
Product is pBjh1601AC-K112321
-----------------------------------------------
JH088F   Forward biobricking of {H-NS!}   
cgataGAATTCatgAGATCTATGAGCGAAGCACTTAAAATTC
JH088R   Reverse biobricking of {H-NS!}   
cgttaGGATCCTTATTGCTTGATCAGGAAATCG


Source

Salmonella L2

References